Journal: Bioengineered

Article Title: LncRNA small nucleolar RNA host gene 5 inhibits trophoblast autophagy in preeclampsia by targeting microRNA-31-5p and promoting the transcription of secreted protein acidic and rich in cysteine

doi: 10.1080/21655979.2022.2040873

Figure Lengend Snippet: SNHG5 is poorly expressed in PE mice, and SNHG5 overexpression alleviates PE phenotype and tissue injury in PE mice. The PE mouse model was established, with healthy mice as the control; and pCDNA3.1-SNHG5 was injected into mice via tail vein, with pCDNA3.1-NC injection as the control. (A) SNHG5 expression pattern in mouse placental tissues was detected by RT-qPCR. (B) proteinuria was extracted from mice using the easy ll Protein Quantitative kits. (C) blood pressure was determined by the tail-cuff technique. (D) levels of TG, TC, LDL, and HDL were measured using the lipid biochemical assay kits. E and F, placenta tissue (e) and kidney tissue (f) were observed by H&E staining. (G) and (H) mRNA expression patterns of Bax (g) and Bcl-2 (h) were assessed by RT-qPCR. The results were presented as mean ± standard deviation. One-way ANOVA was used to analyze the data in panels (A), (B), (G), and (H), and two-way ANOVA was used to analyze the data in panels (C) and (D). Tukey’s multiple comparisons test was applied for the post hoc test. * p < 0.05, ** p < 0.01, *** p < 0.001.

Article Snippet: Blood sample was collected on E17.5 for blood lipid assessment using the UV-Vis spectrophotometry in strict accordance with the provided instructions of the blood lipid biochemical test kits for the following indices: total cholesterol (TC) (Bc1985; Beijing Solarbio Science & Technology Co., Ltd., Beijing China), triglyceride (TG) (BH017Z; Tellgen corporation, Shanghai, China), low-density lipoprotein (LDL) (BH019Z) and high-density lipoprotein (HDL) (BH018Z).

Techniques: Over Expression, Injection, Expressing, Quantitative RT-PCR, Staining, Standard Deviation